Ionic® Purification Kits
Fluidic chips and reagents for the Ionic® Purification System
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Ionic Kits by Application
Ionic Purification Kits and protocols support automated purification of specific sample types and are required for
use with the Ionic Purification System. Purification protocols are developed and validated at Purigen
Biosystems to maximize purification yield and quality with minimal manual steps.
- Ionic® Cells to Pure DNA Kit
![Ionic Cells to Pure DNA Kit]()
Each Cells to Pure DNA Kit contains 6 Ionic Fluidic Chips and reagents to purify DNA from 48 samples.
Sample |
|
| Sample type |
Cultured cells |
| Sample preps per kit |
48 |
| Sample preps per chip |
8 |
Density |
|
| Maximum cell density per sample |
100,000 cells (diploid equivalents) |
| Minimum cell density per sample |
1,000 cells (diploid equivalents) |
Volume |
|
| Input volume per sample prep |
~210 µL of lysate |
| Elution volume per sample prep |
~40 µL |
Run-time |
|
| Run-time per chip |
~60 mins |
Contents |
|
| Fluidic Chip |
6 qty |
| Lysis Buffer 1 |
6 bottles; 1.2 mL |
| Lysis Buffer 2 |
1 bottle; 7.2 mL |
| RNAse Reagent |
1 bottle; 100.0 µL |
| Proteinase K Reagent |
1 bottle; 150.0 µL |
| Extraction Buffer |
1 bottle; 12.0 mL |
| Anodic Buffer |
1 bottle; 12.0 mL |
| Separation Buffer |
1 bottle; 18.0 mL |
| Neutralization Buffer |
1 bottle; 12.0 mL |
| Cathodic Buffer |
1 bottle; 12.0 mL |
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- Ionic® Tissue to Pure DNA Kit
![Ionic Cells to Pure DNA Kit]()
Each Tissue to Pure DNA Kit contains 6 Ionic Fluidic Chips and reagents to purify DNA from 48 samples.
Sample |
|
| Sample type |
Fresh frozen tissue |
| Sample preps per kit |
48 |
| Sample preps per chip |
8 |
Input Amount |
|
| Tissue mass |
Up to 10 mg |
Volume |
|
| Input volume per sample prep |
~200 µL of lysed tissue |
| Extraction volume per sample prep |
~50 µL |
Run-time |
|
| Run-time per chip |
~90 mins |
Contents |
|
| Fluidic Chip |
6 qty |
| Lysis Buffer 1 |
6 bottles; 1.6 mL |
| RNAse A |
1 bottle; 249.0 µL |
| Proteinase K Reagent |
1 bottle; 480.0 µL |
| Tissue Sample Buffer |
1 bottle; 1.6 mL |
| Lysis Buffer 2 |
1 bottle; 4.0 mL |
| Extraction Buffer |
1 bottle; 12.0 mL |
| Anodic Buffer |
1 bottle; 12.0 mL |
| Separation Buffer |
1 bottle; 18.0 mL |
| Neutralization Buffer |
1 bottle; 12.0 mL |
| Cathodic Buffer |
1 bottle; 12.0 mL |
Download Product Info Sheet
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- Ionic® FFPE to Pure DNA Kit
![Ionic FFPE to Pure DNA Kit]()
Each FFPE to Pure DNA Kit contains 6 Ionic Fluidic Chips and reagents to purify DNA from 48
samples.
Sample |
|
| Sample type |
FFPE scroll / tissue section |
| Sample preps per kit |
48 |
| Sample preps per chip |
8 |
Cross-section |
|
| Maximum number of 10 µm thick scrolls/curls |
4 |
| Recommended cross-sectional area per scroll/curl |
50 mm2 – 300 mm2 |
Volume |
|
| Input volume per sample prep |
~200 µL of lysate |
| Elution volume per sample prep |
~40 µL |
Run-time |
|
| Run-time per chip |
~60 mins |
Contents |
|
| Fluidic Chip |
6 qty |
| Lysis Buffer |
6 bottles; 1.8 mL |
| DNAse Reagent |
1 bottle; 250.0 µL |
| Proteinase K Reagent |
1 bottle; 480 µL |
| Extraction Buffer |
1 bottle; 12.0 mL |
| Anodic Buffer |
1 bottle; 12.0 mL |
| Separation Buffer |
6 bottles; 2.5 mL |
| Neutralization Buffer |
1 bottle; 12.0 mL |
| Cathodic Buffer |
1 bottle; 12.0 mL |
| Mineral Oil |
1 bottle; 15.0 mL |
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- Ionic® FFPE to Pure RNA Kit
![Ionic FFPE to Pure RNA Kit]()
Each FFPE to Pure RNA Kit contains 6 Ionic Fluidic Chips and reagents to purify RNA from
48 samples.
Sample |
|
| Sample type |
FFPE scroll / tissue section |
| Sample preps per kit |
48 |
| Sample preps per chip |
8 |
Cross-section |
|
| Maximum number of 10 µm thick scrolls/curls |
2 |
| Recommended cross-sectional area per scroll/curl |
50 mm2 – 300 mm2 |
Volume |
|
| Input volume per sample prep |
~200 µL of lysate |
| Elution volume per sample prep |
~40 µL |
Run-time |
|
| Run-time per chip |
~60 mins |
Contents |
|
| Fluidic Chip |
6 qty |
| Lysis Buffer 1 |
6 bottles; 1.4 mL |
| Lysis Buffer 2 |
1 bottle; 4.0 mL |
| DNAse I Reagent |
1 bottle; 600.0 µL |
| Proteinase K Reagent |
1 bottle; 1.0 mL |
| Extraction Buffer |
1 bottle; 12.0 mL |
| Anodic Buffer |
1 bottle; 12.0 mL |
| Separation Buffer |
6 bottles; 2.5 mL |
| Neutralization Buffer |
1 bottle; 12.0 mL |
| Cathodic Buffer |
1 bottle; 12.0 mL |
| Mineral Oil |
1 bottle; 20.0 mL |
Download Product Info Sheet
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- Ionic® FFPE Complete Kit
![Ionic FFPE to Pure RNA Kit]()
Each FFPE Complete Kit contains 12 Ionic Fluidic Chips and reagents to purify RNA and DNA from 48 samples.
Sample |
|
| Sample type |
FFPE scroll / tissue section |
| Sample preps per kit |
48 (RNA and DNA from each) |
| Sample preps per chip |
8 |
Cross-section |
|
| Maximum number of 10 µm thick scrolls/curls |
2 |
| Recommended cross-sectional area per scroll/curl |
50 mm2 – 300 mm2 |
Volume |
|
| Input volume per sample prep |
~200 µL of lysate |
| Extract volume per sample prep |
~40 µL |
Run-time |
|
| Run-time per chip |
~60 mins |
Contents |
|
| Fluidic Chip |
12 qty |
| Lysis Buffer 1 |
12 bottles; 1.4 mL |
| Lysis Buffer 2 |
1 bottle; 7.0 mL |
| DNase I Reagent |
1 bottle; 700.0 µL |
| RNase Reagent |
1 bottle; 700.0 µL |
| Proteinase K Reagent |
1 bottle; 1.5 mL |
| Extraction Buffer |
1 bottle; 24.0 mL |
| Anodic Buffer |
1 bottle; 24.0 mL |
| Separation Buffer |
2 bottles; 15 mL |
| Neutralization Buffer |
1 bottle; 24.0 mL |
| Cathodic Buffer |
1 bottle; 24.0 mL |
| Mineral Oil |
1 bottle; 20.0 mL |
Download Product Info Sheet
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Purigen Isotachophoresis Technology
Isotachophoresis separates and concentrates charged molecules in solution solely based on their
electrophoretic mobility. Biological samples are gently lysed and added to the Purigen Ionic® Fluidic Chip which applies an electric field to
isolate nucleic acid in its natural, native form.
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