AMP 2022: Analysis of the methylation state of cancer samples is a powerful new tool for research and clinical applications. Ensuring high-quality high-yield DNA extraction from minimal FFPE samples is essential to optimize clinical testing. Here we describe best practices for extraction of DNA from FFPE to support downstream applications including methylation arrays and sequencing. A case study comparing extraction methods for methylation array profiling will be presented. Presented by Dr. Lawrence Jennings at the Northwestern University Feinberg School of Medicine.

AMP 2022: Analysis of the methylation state of cancer samples is a powerful new tool for research and clinical applications. Ensuring high-quality high-yield DNA extraction from minimal FFPE samples is essential to optimize clinical testing. Here we describe best practices for extraction of DNA from FFPE to support downstream applications including methylation arrays and sequencing. A case study comparing extraction methods for methylation array profiling will be presented. Presented by Dr. Lawrence Jennings at the Northwestern University Feinberg School of Medicine.

ABRF 2022 Technology Showcase with special guest speaker Dr. Jian Gu from the University of Texas, MD Anderson Cancer Center. This video discusses how the next-gen Ionic® Purification System enables pure nucleic acid extraction from FFPE samples using our proprietary charge-based isotachophoresis (ITP) technology.

Dr. Jian Gu from The University of Texas, MD Anderson Cancer Center discusses how the next-gen Ionic® Purification System Improves core lab operations and increases customer satisfaction.

Peripheral Blood Mononuclear Cells (PBMCs) are a crucial sample type for a broad range of research applications including profiling immune cells and serving as “normal” controls for studies of FFPE tumor tissue for oncology studies. Maximizing the DNA yield from PBMCs enables archiving and increases the number of downstream assays that can be performed. The Ionic Purification System from Purigen Biosystems leverages isotachophoresis to maximize the yield of DNA from PBMCs. During the webinar data will be presented showing significantly improved yields of DNA extracted from PBMCs ranging in cell count from 10 cells to 5 million cells. Learning Objectives: • Learn how DNA yield and quality impact downstream genomic analysis • Understand how Isotachophoresis (ITP) works and enables superior nucleic acid extraction vs legacy technologies (columns, beads, precipitation, etc.) • Evaluate data showing superior yields from ITP-based extraction • Explore options for trying out ITP in your lab

The new Ionic® FFPE Complete Purification Kit from Purigen Biosystems provides simultaneous purification of DNA and RNA, including miRNA, from FFPE tissues. While most technologies use a serial approach to separately isolate DNA and RNA, this new kit enables a single, automated workflow using the Ionic® Purification System that requires 75% less hands on time.